Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (ER1917-44) at 1:800 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(ER1917-44), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: Bulbus olfactorius (Rat) Lysate at 40 ug Primary: Anti-Synaptoporin (ER1917-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 28 kD| Product Name | SYNPR/Synaptoporin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPO Synaptophysin 2 Synaptoporin Synpr SYNPR_HUMAN DKFZp686G0883 MGC26651. |
| Product description | Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein. |
| Immunogen | KLH conjugated synthetic peptide derived from human Synaptoporin 31-130/265 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | SPO Synaptophysin 2 Synaptoporin Synpr SYNPR_HUMAN DKFZp686G0883 MGC26651. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. |
| SwissProt ID | Q8TBG9 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (ER1917-44) at 1:800 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(ER1917-44), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: Bulbus olfactorius (Rat) Lysate at 40 ug Primary: Anti-Synaptoporin (ER1917-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 28 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
