Fig1: Sample:
U2OS Cell Lysate at 40 ug
Hela Cell Lysate at 40 ug
Primary: Anti-TAF1A (ER1917-47) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
TAF1 48
MGC:17061
RAFI48
RNA polymerase I-specific TBP-associated factor 48 kDa
SL1 48kD subunit
SL1
Taf1a
TAF1A_HUMAN
TAFI48
TATA box binding protein (TBP) associated factor RNA polymerase I, A 48kDa
TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A
TATA box binding protein associated factor 1A
TATA box binding protein associated factor RNA polymerase I subunit A
TATA box-binding protein-associated factor 1A
TATA box-binding protein-associated factor RNA polymerase I subunit A
TBP associated factor RNA polymerase I 48 kDa
TBP-associated factor 1A
Transcription factor SL1
Transcription initiation factor SL1/TIF-IB subunit A.
Product description
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
Immunogen
KLH conjugated synthetic peptide derived from human TAF1A 21-120/450
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500-2000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Target Information
Alternative Names
TAF1 48
MGC:17061
RAFI48
RNA polymerase I-specific TBP-associated factor 48 kDa
SL1 48kD subunit
SL1
Taf1a
TAF1A_HUMAN
TAFI48
TATA box binding protein (TBP) associated factor RNA polymerase I A 48kDa
TATA box binding protein (Tbp)-associated factor RNA polymerase I A
TATA box binding protein associated factor 1A
TATA box binding protein associated factor RNA polymerase I subunit A
TATA box-binding protein-associated factor 1A
TATA box-binding protein-associated factor RNA polymerase I subunit A
TBP associated factor RNA polymerase I 48 kDa
TBP-associated factor 1A
Transcription factor SL1
Transcription initiation factor SL1/TIF-IB subunit A.
Fig1: Sample:
U2OS Cell Lysate at 40 ug
Hela Cell Lysate at 40 ug
Primary: Anti-TAF1A (ER1917-47) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Application Notes
WB:1:500-2000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.