Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TATA binding protein TBP/TBP) Polyclonal Antibody, Unconjugated (ER1917-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti- TATA binding protein TBP/TBP (ER1917-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kD| Product Name | TATA binding protein TBP/TBP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTF2D GTF2D1 TFIID MGC117320 MGC126054 MGC126055 SCA 17 SCA17 TATA binding factor TATA box binding protein TATA box factor TATA sequence binding protein TATA sequence-binding protein TATA binding protein TBP TATA-binding factor TATA-box factor TATA-box-binding protein TBP TBP_HUMAN TF2D TFIID Transcription initiation factor TFIID TBP subunit. |
| Product description | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
| Immunogen | KLH conjugated synthetic peptide derived from human TATA binding protein TBP 201-339/339 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | GTF2D GTF2D1 TFIID MGC117320 MGC126054 MGC126055 SCA 17 SCA17 TATA binding factor TATA box binding protein TATA box factor TATA sequence binding protein TATA sequence-binding protein TATA binding protein TBP TATA-binding factor TATA-box factor TATA-box-binding protein TBP TBP_HUMAN TF2D TFIID Transcription initiation factor TFIID TBP subunit. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P20226 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TATA binding protein TBP/TBP) Polyclonal Antibody, Unconjugated (ER1917-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti- TATA binding protein TBP/TBP (ER1917-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kD| Positive Control | Widely expressed, with levels highest in the testis and ovary. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
