Application
Fig1: Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- SMAP55 (ER1917-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TBL1X) Polyclonal Antibody, Unconjugated (ER1917-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | TBL1X/SMAP55 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBL1 EBI F box like/WD repeat protein TBL1X SMAP 55 SMAP55 TBL 1 TBL1X Transducin (beta) like 1 Transducin (beta) like 1 X linked Transducin beta like 1 X linked Transducin beta like 1X Transducin beta like 1X protein TBL1X_HUMAN F-box-like/WD repeat-containing protein TBL1X Full=Transducin beta-like protein 1X Transducin-beta-like protein 1, X-linked. . |
| Product description | F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange. |
| Immunogen | KLH conjugated synthetic peptide derived from human TBL1X 501-577/577 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | TBL1
EBI
F box like/WD repeat protein TBL1X
SMAP 55
SMAP55
TBL 1
TBL1X
Transducin (beta) like 1
Transducin (beta) like 1 X linked
Transducin beta like 1 X linked
Transducin beta like 1X
Transducin beta like 1X protein
TBL1X_HUMAN
F-box-like/WD repeat-containing protein TBL1X
Full=Transducin beta-like protein 1X
Transducin-beta-like protein 1 X-linked. . |
|---|---|
| Molecular Weight(MW) | 63 KDa |
| Cellular Localization | Nucleus (Probable). |
| SwissProt ID | O60907 |
|---|
Application
Fig1: Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- SMAP55 (ER1917-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TBL1X) Polyclonal Antibody, Unconjugated (ER1917-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
