Application
Fig1: Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kDApplication
Fig2: Sample: SW480(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kDApplication
Fig3: Sample: spleen(mouse) Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kDApplication
Fig4: Sample:Thymus(mouse) Lysate at 40 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (ER1917-58) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | TEM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tumor endothelial marker 1 precursor TEM 1 TEM-1 tumor endothelial marker 1 CD248 Endosialin Tumor endothelial marker 1 CD164L1 MGC119478 MGC119479. CD164 sialomucin-like 1 CD248_HUMAN. |
| Product description | May play a role in tumor angiogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human TEM 1 251-350/757 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Tumor endothelial marker 1 precursor TEM 1 TEM-1 tumor endothelial marker 1 CD248 Endosialin Tumor endothelial marker 1 CD164L1 MGC119478 MGC119479. CD164 sialomucin-like 1 CD248_HUMAN. |
|---|---|
| Molecular Weight(MW) | 80 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Probable). |
| SwissProt ID | Q9HCU0 |
|---|
Application
Fig1: Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kD
Application
Fig2: Sample: SW480(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kD
Application
Fig3: Sample: spleen(mouse) Lysate at 30 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kD
Application
Fig4: Sample:Thymus(mouse) Lysate at 40 ug Primary: Anti-TEM1 (ER1917-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 130 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TEM1) Polyclonal Antibody, Unconjugated (ER1917-58) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
