Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- TEM8 (ER1917-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 62 kDApplication
Fig2: Sample: Siha(Human) Cell Lysate at 30 ug Uterus (Mouse) Lysate at 40 ug Primary: Anti- TEM8 (ER1917-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 62 kD| Product Name | TEM8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Anthrax toxin receptor 1 Anthrax toxin receptor 1 precursor ANTXR 1 ANTXR1 ATR TEM-8 TEM 8 TEM8 Tumor Endothelial Marker 8 Tumor endothelial marker 8 precursor ANTR1_HUMAN Anthrax toxin receptor-1 FLJ11298 FLJ21776. |
| Product description | Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human TEM8 201-300/564 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Anthrax toxin receptor 1 Anthrax toxin receptor 1 precursor ANTXR 1 ANTXR1 ATR TEM-8 TEM 8 TEM8 Tumor Endothelial Marker 8 Tumor endothelial marker 8 precursor ANTR1_HUMAN Anthrax toxin receptor-1 FLJ11298 FLJ21776. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein. Note=At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia. |
| SwissProt ID | Q9H6X2 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- TEM8 (ER1917-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 62 kD
Application
Fig2: Sample: Siha(Human) Cell Lysate at 30 ug Uterus (Mouse) Lysate at 40 ug Primary: Anti- TEM8 (ER1917-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 62 kD| Positive Control | Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
