Application
Fig1: Tissue/cell: mouse small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(ER1917-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.Application
Fig2: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(ER1917-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Cell:HT-29 Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of primary antibody (Cat#: ER1917-60) on HT-29(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .Application
Fig4: Sample: Small intestine (Mouse) Lysate at 40 ug Small intestine (Rat) Lysate at 40 ug Primary: Anti-TFF3 (ER1917-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 6.5 kD Observed band size: 16 kD| Product Name | TFF3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Trefoil Factor 3 Trefoil factor3 hP1.B Intestinal trefoil factor ITF mITF OTTMUSP00000021729 Polypeptide P1.B TFF3 TFI TREFOIL Trefoil factor (intestinal). |
| Immunogen | KLH conjugated synthetic peptide derived from human TFF3 221-320/923 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | Trefoil Factor 3 Trefoil factor3 hP1.B Intestinal trefoil factor ITF mITF OTTMUSP00000021729 Polypeptide P1.B TFF3 TFI TREFOIL Trefoil factor (intestinal). |
|---|---|
| Molecular Weight(MW) | 6.5 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, apical lamina. |
| SwissProt ID | Q07654 |
|---|
Application
Fig1: Tissue/cell: mouse small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(ER1917-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
Application
Fig2: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(ER1917-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Cell:HT-29 Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of primary antibody (Cat#: ER1917-60) on HT-29(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Application
Fig4: Sample: Small intestine (Mouse) Lysate at 40 ug Small intestine (Rat) Lysate at 40 ug Primary: Anti-TFF3 (ER1917-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 6.5 kD Observed band size: 16 kD| Positive Control | Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
