Application
Fig1: Sample:Kidney(Mouse)Lysate at 40 ug Primary: Anti-TGF beta 2 Propeptide(ER1917-63)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12'45kD Observed band size: 50 kDApplication
Fig2: Sample:MCF-7 (Human)Cell Lysate at 40 ug Primary: Anti-TGF beta 2 Propeptide(ER1917-63)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12'45kD Observed band size: 48kD| Product Name | TGF beta 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BSC 1 cell growth inhibitor BSC1 cell growth inhibitor Cetermin G TSF Glioblastoma derived T cell suppressor Glioblastoma derived T cell suppressor factor GTSF MGC116892 Polyergin TGF B2 TGF beta2 TGFB 2 TGFB2 Transforming growth factor beta 2.TGF B2 BSC 1 cell growth inhibitor BSC-1 cell growth inhibitor BSC1 cell growth inhibitor Cetermin G TSF G-TSF Glioblastoma derived T cell suppressor Glioblastoma derived T cell suppressor factor Glioblastoma-derived T-cell suppressor factor GTSF MGC116892 Polyergin TGF beta2 TGF-beta-2 TGF-beta2 TGFB 2 TGFB2 TGFB2_HUMAN Transforming growth factor beta 2 Transforming growth factor beta-2. |
| Product description | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Immunogen | KLH conjugated synthetic peptide derived from human TGF-beta 2 154-197/414 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | BSC 1 cell growth inhibitor
BSC1 cell growth inhibitor
Cetermin G TSF Glioblastoma derived T cell suppressor Glioblastoma derived T cell suppressor factor GTSF MGC116892 Polyergin TGF B2 TGF beta2 TGFB 2 TGFB2 Transforming growth factor beta 2.TGF B2 BSC 1 cell growth inhibitor BSC-1 cell growth inhibitor BSC1 cell growth inhibitor Cetermin G TSF G-TSF Glioblastoma derived T cell suppressor Glioblastoma derived T cell suppressor factor Glioblastoma-derived T-cell suppressor factor GTSF MGC116892 Polyergin TGF beta2 TGF-beta-2 TGF-beta2 TGFB 2 TGFB2 TGFB2_HUMAN Transforming growth factor beta 2 Transforming growth factor beta-2. |
|---|---|
| Molecular Weight(MW) | 12/45 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P61812 |
|---|
Application
Fig1: Sample:Kidney(Mouse)Lysate at 40 ug Primary: Anti-TGF beta 2 Propeptide(ER1917-63)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12'45kD Observed band size: 50 kD
Application
Fig2: Sample:MCF-7 (Human)Cell Lysate at 40 ug Primary: Anti-TGF beta 2 Propeptide(ER1917-63)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 12'45kD Observed band size: 48kD| Positive Control | Kidney (Mouse) Lysate, MCF-7 (Human) Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
