Application
Fig1: Tissue/cell: pig bile duct; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated(ER1917-64) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Placenta (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-TGF beta 3 propeptide (ER1917-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/47 kD Observed band size: 55 kD| Product Name | TGF beta 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TGF beta 3 ARVD FLJ16571 MGC105479 MGC118722 TGF beta 3 TGF beta3 TGF-beta-3 TGF-beta 3 TGFB 3 TGFB3 TGFB3_HUMAN transforming growth factor beta 3 Transforming growth factor beta-3. |
| Product description | Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3. Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. |
| Immunogen | KLH conjugated synthetic peptide derived from human TGF-beta 3 41-90/412 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | TGF beta 3
ARVD
FLJ16571 MGC105479 MGC118722 TGF beta 3 TGF beta3 TGF-beta-3 TGF-beta 3 TGFB 3 TGFB3 TGFB3_HUMAN transforming growth factor beta 3 Transforming growth factor beta-3. |
|---|---|
| Molecular Weight(MW) | 30/47 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P10600 |
|---|
Application
Fig1: Tissue/cell: pig bile duct; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated(ER1917-64) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Placenta (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-TGF beta 3 propeptide (ER1917-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/47 kD Observed band size: 55 kD| Positive Control | Pig bile duct, Mouse Placenta Lysate, Mouse Muscle Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
