Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-TGF beta Receptor II (ER1917-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kDApplication
Fig2: Sample: Brain(Mouse) Lysate at 30 ug Brain(Rat) Lysate at 30 ug Primary: Anti-TGF-beta-R2/TGFBR2 (ER1917-66) at 1/300 dilution Secondary:IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kDApplication
Fig3: Sample: HepG2 Cell Lysate at 40 ug Primary: Anti- TGF beta RECEPTOR Ⅱ(ER1917-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD| Product Name | TGF beta Receptor II |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TGF beta R2 TGFBR2 TGF beta Receptor II AAT 3 AAT3 FAA 3 FAA3 HNPCC6 MFS 2 MFS2 RIIC TAAD 2 TAAD2 TbetaR II TGF beta receptor type 2 TGF beta receptor type II TGF beta type II receptor TGFB R2 TGFbeta RII TGFBR 2 TGFBR2 TGFR 2 TGFR2 Transforming growth factor beta receptor II Transforming growth factor beta receptor type II |
| Product description | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. |
| Immunogen | KLH conjugated synthetic peptide derived from human TGF beta R2 241-330/567 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | TGF beta R2 TGFBR2 TGF beta Receptor II AAT 3 AAT3 FAA 3 FAA3 HNPCC6 MFS 2 MFS2 RIIC TAAD 2 TAAD2 TbetaR II TGF beta receptor type 2 TGF beta receptor type II TGF beta type II receptor TGFB R2 TGFbeta RII TGFBR 2 TGFBR2 TGFR 2 TGFR2 Transforming growth factor beta receptor II Transforming growth factor beta receptor type II |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
| SwissProt ID | P37173 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-TGF beta Receptor II (ER1917-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD
Application
Fig2: Sample: Brain(Mouse) Lysate at 30 ug Brain(Rat) Lysate at 30 ug Primary: Anti-TGF-beta-R2/TGFBR2 (ER1917-66) at 1/300 dilution Secondary:IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD
Application
Fig3: Sample: HepG2 Cell Lysate at 40 ug Primary: Anti- TGF beta RECEPTOR Ⅱ(ER1917-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD| Positive Control | Kidney (Mouse) Lysate, Brain(Mouse) Lysate, Brain(Rat) Lysate, HepG2 Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
