Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Embryo(Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-Thrombin Activation peptide fragment 1 (ER1917-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/64 kD Observed band size: 17/64 kD| Product Name | Thrombin Activation peptide fragment 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activation peptide fragment 1 coagulation factor II prothrombin F2 Cf-2 Cf2 FII F 2 coagulation factor II (thrombin) Coagulation factor II Coagulation factor II precursor F2 Factor II Factor-II Prothrombin prothrombin B-chain PT serine protease THRB THRB_HUMAN Thrombin. |
| Product description | Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Gamma Thrombin is a proteolyzed form of Alpha Thrombin. Gamma Thrombin consists of four chains (A, B1, B5, B4) with a disulfide link between the A peptide and the B5 peptide. |
| Immunogen | KLH conjugated synthetic peptide derived from human Thrombin Activation peptide fragment 1 51-150/622 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Activation peptide fragment 1 coagulation factor II prothrombin F2 Cf-2 Cf2 FII F 2 coagulation factor II (thrombin) Coagulation factor II Coagulation factor II precursor F2 Factor II Factor-II Prothrombin prothrombin B-chain PT serine protease THRB THRB_HUMAN Thrombin. |
|---|---|
| Molecular Weight(MW) | 17/64 KDa |
| Cellular Localization | Secreted, extracellular space. |
| SwissProt ID | P00734 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Embryo(Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-Thrombin Activation peptide fragment 1 (ER1917-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/64 kD Observed band size: 17/64 kD| Positive Control | Expressed by the liver and secreted in plasma. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
