Application
Fig1: Protein: liver(mouse) lysate at 40ug; Primary: rabbit Anti-Thrombin light chain (ER1917-71) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig2: Protein: placenta(mouse) lysate at 40ug; Primary: rabbit Anti-Thrombin light chain (ER1917-71) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig3: Sample: placenta (Mouse) Lysate at 40 ug Primary: Anti-Thrombin light chain(ER1917-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Product Name | Thrombin light chain |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | coagulation factor II prothrombin F2 Cf-2 Cf2 FII F 2 coagulation factor II (thrombin) Coagulation factor II Coagulation factor II precursor F2 Factor II Factor-II Prothrombin prothrombin B-chain PT serine protease THRB THRB_HUMAN Thrombin Thrombin light chain. |
| Product description | Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Gamma Thrombin is a proteolyzed form of Alpha Thrombin. Gamma Thrombin consists of four chains (A, B1, B5, B4) with a disulfide link between the A peptide and the B5 peptide. |
| Immunogen | KLH conjugated synthetic peptide derived from human Thrombin light chain 328-363/622 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | coagulation factor II prothrombin F2 Cf-2 Cf2 FII F 2 coagulation factor II (thrombin) Coagulation factor II Coagulation factor II precursor F2 Factor II Factor-II Prothrombin prothrombin B-chain PT serine protease THRB THRB_HUMAN Thrombin Thrombin light chain. |
|---|---|
| Molecular Weight(MW) | 4/64 KDa |
| Cellular Localization | Secreted, extracellular space. |
| SwissProt ID | P00734 |
|---|
Application
Fig1: Protein: liver(mouse) lysate at 40ug; Primary: rabbit Anti-Thrombin light chain (ER1917-71) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig2: Protein: placenta(mouse) lysate at 40ug; Primary: rabbit Anti-Thrombin light chain (ER1917-71) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig3: Sample: placenta (Mouse) Lysate at 40 ug Primary: Anti-Thrombin light chain(ER1917-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Positive Control | Expressed by the liver and secreted in plasma. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
