Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (ER1917-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-PAR1 (ER1917-72) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (ER1917-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig4: Sample: Brain (Rat) Lysate at 30 ug Liver (Mouse) Lysate at 30 ug Primary: Anti- PAR-1 (ER1917-72) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 42kD Observed band size : 44kDApplication
Fig5: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(ER1917-72) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig6: Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(ER1917-72) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | Thrombin receptor/ PAR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAR BXR Proteinase-activated receptor 1 precursor Thrombin receptor Coagulation factor II receptor D15S227E Prader-Willi/Angelman region-1 PAR1 Coagulation factor II receptor CF2R Coagulation factor II (thrombin) receptor F2R HTR PAR 1 PAR1 Protease activated receptor 1 Proteinase activated receptor 1 Thrombin receptor TR PAR-1 Coagulation factor II receptor Coagulation factor II receptor PAR-1 PAR1_HUMAN Proteinase activated receptor 1 Proteinase-activated receptor 1. |
| Product description | High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. |
| Immunogen | KLH conjugated synthetic peptide derived from human PAR1 251-350/425 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | SAR
BXR
Proteinase-activated receptor 1 precursor Thrombin receptor Coagulation factor II receptor D15S227E Prader-Willi/Angelman region-1 PAR1 Coagulation factor II receptor CF2R Coagulation factor II (thrombin) receptor F2R HTR PAR 1 PAR1 Protease activated receptor 1 Proteinase activated receptor 1 Thrombin receptor TR PAR-1 Coagulation factor II receptor Coagulation factor II receptor PAR-1 PAR1_HUMAN Proteinase activated receptor 1 Proteinase-activated receptor 1. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P25116 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (ER1917-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-PAR1 (ER1917-72) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (ER1917-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig4: Sample: Brain (Rat) Lysate at 30 ug Liver (Mouse) Lysate at 30 ug Primary: Anti- PAR-1 (ER1917-72) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 42kD Observed band size : 44kD
Application
Fig5: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(ER1917-72) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig6: Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(ER1917-72) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Platelets and vascular endothelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
