Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (ER1917-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (ER1917-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-thymidine phosphorylase (ER1917-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Product Name | thymidine phosphorylase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECGF 1 PDEGF ECGF1 Endothelial cell growth factor 1 Endothelial cell growth factor 1 platelet derived Gliostatin hPD ECGF MNGIE PD ECGF PDECGF PDEGF Platelet derived endothelial growth factor TdRPase TP TYMP Thymidine Phosphorylase TYPH_HUMAN TdRPase. |
| Product description | May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. ;Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase 121-220/482 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ECGF 1 PDEGF ECGF1 Endothelial cell growth factor 1 Endothelial cell growth factor 1 platelet derived Gliostatin hPD ECGF MNGIE PD ECGF PDECGF PDEGF Platelet derived endothelial growth factor TdRPase TP TYMP Thymidine Phosphorylase TYPH_HUMAN TdRPase. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| SwissProt ID | P19971 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (ER1917-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (ER1917-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-thymidine phosphorylase (ER1917-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
