Application
Fig1: Sample: Liver (Rat) Lysate at 40 ug Primary: Anti- Thyroid hormone receptor alpha (ER1917-76) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Thyroid hormone receptor alpha (ER1917-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig3: Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Thyroid hormone receptor alpha antibody(ER1917-76), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Thyroid hormone receptor alpha (ER1917-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | Thyroid hormone receptor alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AR7 c-erbA-1 C-erbA-alpha CHNG6 EAR 7 EAR-7 EAR7 ERB-T-1 ERBA alpha ERBA ERBA Related 7 ERBA-related 7 ERBA1 NR1A1 Nuclear receptor subfamily 1 group A member 1 THA_HUMAN THRA 1 THRA THRA1 THRA2 Thyroid hormone receptor alpha thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) thyroid normone nuclear receptor alpha variant 1 triiodothyronine receptor V-erbA-related protein 7. |
| Product description | Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. |
| Immunogen | KLH conjugated synthetic peptide derived from human Thyroid hormone receptor alpha 81-180/490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenPigHorseRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | AR7
c-erbA-1
C-erbA-alpha
CHNG6
EAR 7
EAR-7
EAR7
ERB-T-1
ERBA alpha
ERBA
ERBA Related 7
ERBA-related 7
ERBA1
NR1A1
Nuclear receptor subfamily 1 group A member 1
THA_HUMAN
THRA 1
THRA
THRA1
THRA2
Thyroid hormone receptor alpha
thyroid hormone receptor alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog avian) thyroid normone nuclear receptor alpha variant 1 triiodothyronine receptor V-erbA-related protein 7. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P10827 |
|---|
Application
Fig1: Sample: Liver (Rat) Lysate at 40 ug Primary: Anti- Thyroid hormone receptor alpha (ER1917-76) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Thyroid hormone receptor alpha (ER1917-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig3: Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Thyroid hormone receptor alpha antibody(ER1917-76), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Thyroid hormone receptor alpha (ER1917-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | After the mid-blastula transition (MBT), expressed throughout the deep cells, which give rise to the embryo proper. In adults, isoform 2 shows highest expression in the eye and liver. Expressed in adult gonads. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
