Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-TKTL1 (ER1917-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-TKTL1 (ER1917-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (ER1917-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (ER1917-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | TKTL1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TK 2 TK2 TKR TKT 2 TKT2 TKTL1 TKTL1_HUMAN Transketolase 2 Transketolase like 1 Transketolase like protein 1 Transketolase related protein Transketolase-like protein 1 Transketolase-related protein Transketolase2. |
| Product description | Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate |
| Immunogen | KLH conjugated synthetic peptide derived from human TKTL1 101-200/596 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | TK 2 TK2 TKR TKT 2 TKT2 TKTL1 TKTL1_HUMAN Transketolase 2 Transketolase like 1 Transketolase like protein 1 Transketolase related protein Transketolase-like protein 1 Transketolase-related protein Transketolase2. |
|---|---|
| Molecular Weight(MW) | 65 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic and to a lesser extent also nuclear. |
| SwissProt ID | P51854 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-TKTL1 (ER1917-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-TKTL1 (ER1917-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (ER1917-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TKTL1) Polyclonal Antibody, Unconjugated (ER1917-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
