Application
peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM49 Polyclonal Antibody, Unconjugated(ER1917-82) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-TMEM49(ER1917-82) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Product Name | TMEM49 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vacuole membrane protein 1 DKFZP566I133 TDC1 TMEM49 Transmembrane protein 49 vmp1 VMP1_HUMAN. |
| Product description | Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1 (By similarity). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions.Sequence similarities: Belongs to the VMP1 family. |
| Immunogen | KLH conjugated synthetic peptide derived from human TMEM49 361-406/406 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Vacuole membrane protein 1 DKFZP566I133 TDC1 TMEM49 Transmembrane protein 49 vmp1 VMP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. Vacuole membrane. Endoplasmic reticulum. |
| SwissProt ID | Q96GC9 |
|---|
Application
peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM49 Polyclonal Antibody, Unconjugated(ER1917-82) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-TMEM49(ER1917-82) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
