Application
Fig1: Sample: HEK293T KO TMP21(Human) Cell Lysate at 30 ug HEK293T(Human) Cell Lysate at 30 ug Primary: Anti-TMP21 (ER1917-85) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (ER1917-85) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | TMP21 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p23 p24 family protein delta 1 p24delta p24delta1 S31I125 S31III125 TMED 10 TMED10 Tmp 21 I Tmp 21 Tmp 21 I Transmembrane emp24 domain containing protein 10 Transmembrane emp24-like trafficking protein 10 (yeast) Transmembrane protein Tmp21 Transmembrane trafficking protein 21kD 1110014C03Rik 21 kDa transmembrane trafficking protein MGC102351 TMEDA_HUMAN. |
| Product description | Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. |
| Immunogen | KLH conjugated synthetic peptide derived from human TMP21 101-200/219 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | p23 p24 family protein delta 1 p24delta p24delta1 S31I125 S31III125 TMED 10 TMED10 Tmp 21 I Tmp 21 Tmp 21 I Transmembrane emp24 domain containing protein 10 Transmembrane emp24-like trafficking protein 10 (yeast) Transmembrane protein Tmp21 Transmembrane trafficking protein 21kD 1110014C03Rik 21 kDa transmembrane trafficking protein MGC102351 TMEDA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Melanosome. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type I membrane protein (By similarity). Cell membrane (By similarity). Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway. |
| SwissProt ID | P49755 |
|---|
Application
Fig1: Sample: HEK293T KO TMP21(Human) Cell Lysate at 30 ug HEK293T(Human) Cell Lysate at 30 ug Primary: Anti-TMP21 (ER1917-85) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (ER1917-85) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
