Application
Fig1: Sample: bone(mouse) Lysate at 40 ug Primary: Anti-TNFSF15 (ER1917-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 38 kDApplication
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TNFSF15 Polyclonal Antibody, Unconjugated(ER1917-90) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | TNFSF15 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TL1A TL1 A TL 1A TL-1A tl1 tl1a tnf ligand related molecule 1 TNF ligand-related molecule 1 tnf superfamily ligand tl1a TNF15_HUMAN Tumor necrosis factor ligand superfamily member 15 Vascular endothelial cell growth inhibitor Vascular endothelial growth inhibitor 192a Vascular endothelial growth inhibitor vegi VEGI192A antibodysecreted form MGC129934 MGC129935. |
| Product description | Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human TNFSF15 161-251/251 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | TL1A TL1 A TL 1A TL-1A tl1 tl1a tnf ligand related molecule 1 TNF ligand-related molecule 1 tnf superfamily ligand tl1a TNF15_HUMAN Tumor necrosis factor ligand superfamily member 15 Vascular endothelial cell growth inhibitor Vascular endothelial growth inhibitor 192a Vascular endothelial growth inhibitor vegi VEGI192A antibodysecreted form MGC129934 MGC129935. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein (Probable). Tumor necrosis factor ligand superfamily member 15, secreted form: Secreted. |
| SwissProt ID | O95150 |
|---|
Application
Fig1: Sample: bone(mouse) Lysate at 40 ug Primary: Anti-TNFSF15 (ER1917-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 38 kD
Application
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TNFSF15 Polyclonal Antibody, Unconjugated(ER1917-90) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
