Application
Fig1: Sample: Lung (Mouse) Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: rabbit Anti- TRAIL (ER1917-99) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution; Predicted band size: 31 kD Observed band size: 26 kDApplication
Fig2: Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(ER1917-99) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(ER1917-99) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | TRAIL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APO2L TNFSF10 Apo 2 ligand Apo 2L CD 253 CD253 CD253 antigen Protein TRAIL TL 2 TL2 TNF Related Apoptosis Inducing Ligand TNF related apoptosis inducing ligand TRAIL TNFSF 10 TNFSF10 protein TRAIL TRAIL PEN Tumor Necrosis Factor (ligand) Superfamily Member 10 TNF10_HUMAN. |
| Product description | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRAIL 185-281/281 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | APO2L TNFSF10 Apo 2 ligand Apo 2L CD 253 CD253 CD253 antigen Protein TRAIL TL 2 TL2 TNF Related Apoptosis Inducing Ligand TNF related apoptosis inducing ligand TRAIL TNFSF 10 TNFSF10 protein TRAIL TRAIL PEN Tumor Necrosis Factor (ligand) Superfamily Member 10 TNF10_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein (Potential). |
| SwissProt ID | P50591 |
|---|
Application
Fig1: Sample: Lung (Mouse) Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: rabbit Anti- TRAIL (ER1917-99) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution; Predicted band size: 31 kD Observed band size: 26 kD
Application
Fig2: Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(ER1917-99) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(ER1917-99) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Widespread; most predominant in spleen, lung and prostate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
