Application
Fig1: Blank control (blue line): Hela(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-TRAP80 antibody (ER1918-02),ilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.Application
Fig2: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kDApplication
Fig3: Sample: spleen(mouse) Lysate at 40 ug Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73kD Observed band size: 75 kD| Product Name | TRAP80 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MED17 CRSP77 Activator recruited cofactor 77 kDa component Activator-recruited cofactor 77 kDa component ARC77 Cofactor required for Sp1 transcriptional activation subunit 6 CRSP complex subunit 6 CRSP6 CRSP77 DRIP80 med17 MED17_HUMAN Mediator complex subunit 17 Mediator of RNA polymerase II transcription subunit 17 Thyroid hormone receptor-associated protein complex 80 kDa component Transcriptional coactivator CRSP77 Trap80 Vitamin D3 receptor-interacting protein complex 80 kDa component. |
| Product description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRAP80 181-280/651 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | MED17 CRSP77 Activator recruited cofactor 77 kDa component Activator-recruited cofactor 77 kDa component ARC77 Cofactor required for Sp1 transcriptional activation subunit 6 CRSP complex subunit 6 CRSP6 CRSP77 DRIP80 med17 MED17_HUMAN Mediator complex subunit 17 Mediator of RNA polymerase II transcription subunit 17 Thyroid hormone receptor-associated protein complex 80 kDa component Transcriptional coactivator CRSP77 Trap80 Vitamin D3 receptor-interacting protein complex 80 kDa component. |
|---|---|
| Molecular Weight(MW) | 73 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9NVC6 |
|---|
Application
Fig1: Blank control (blue line): Hela(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃ and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-TRAP80 antibody (ER1918-02),ilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Application
Fig2: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kD
Application
Fig3: Sample: spleen(mouse) Lysate at 40 ug Primary: Anti-TRAP80 (ER1918-02) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73kD Observed band size: 75 kD| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
