Application
Fig1: Sample: U937 Cell Lysate at 40 ug Primary: Anti-TRIM21 (ER1918-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 56 kDApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- TRIM21 (ER1918-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- TRIM21 (ER1918-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Product Name | TRIM21 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 52 kDa ribonucleoprotein autoantigen Ro/SS-A 52 kDa Ro protein 52kD Ro/SSA autoantigen Autoantigen Ro/SSA, 52-KD E3 ubiquitin-protein ligase TRIM21 RING finger protein 81 RNF81 Ro 52 Ro(SS-A) Ro52 RO52_HUMAN Sicca syndrome antigen A Sjoegren syndrome type A antigen Sjogren syndrome antigen A1 Sjogren syndrome type A antigen SS-A SSA SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) TRIM21 Tripartite motif protein TRIM21 Tripartite motif-containing 21 Tripartite motif-containing protein 21. |
| Product description | E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. |
| Immunogen | KLH conjugated synthetic peptide derived from human SSA N-terminus 301-400/475 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 52 kDa ribonucleoprotein autoantigen Ro/SS-A
52 kDa Ro protein
52kD Ro/SSA autoantigen
Autoantigen Ro/SSA 52-KD E3 ubiquitin-protein ligase TRIM21 RING finger protein 81 RNF81 Ro 52 Ro(SS-A) Ro52 RO52_HUMAN Sicca syndrome antigen A Sjoegren syndrome type A antigen Sjogren syndrome antigen A1 Sjogren syndrome type A antigen SS-A SSA SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) TRIM21 Tripartite motif protein TRIM21 Tripartite motif-containing 21 Tripartite motif-containing protein 21. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P19474 |
|---|
Application
Fig1: Sample: U937 Cell Lysate at 40 ug Primary: Anti-TRIM21 (ER1918-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 56 kD
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- TRIM21 (ER1918-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig3: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- TRIM21 (ER1918-07) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Positive Control | Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
