Application
Fig1: Sample: Raji Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-Trim22 (ER1918-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Product Name | Trim22 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 50 kDa stimulated trans acting factor RING finger protein 94 RNF94 Staf-50 RNF94 Staf 50 STAF50 Stimulated trans acting factor (50 kDa) Stimulated Trans Acting Factor (homolog of Mouse Rpt 1 gene) Trim22 Tripartite binding motif 22 Tripartite motif containing protein 22 TTRIM22RIM22 TRI22_HUMAN. |
| Product description | Trim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms. |
| Immunogen | KLH conjugated synthetic peptide derived from Human Trim22/Staf-50/RNF94 231-330/498 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 50 kDa stimulated trans acting factor RING finger protein 94 RNF94 Staf-50 RNF94 Staf 50 STAF50 Stimulated trans acting factor (50 kDa) Stimulated Trans Acting Factor (homolog of Mouse Rpt 1 gene) Trim22 Tripartite binding motif 22 Tripartite motif containing protein 22 TTRIM22RIM22 TRI22_HUMAN. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q8IYM9 |
|---|
Application
Fig1: Sample: Raji Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-Trim22 (ER1918-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD| Positive Control | Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
