Application
Fig1: Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-TRIM23(ER1918-09)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig2: Sample: Hela (Human) Cell Lysate at 40 ug Primary: Anti-TRIM23(ER1918-09)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM23) Polyclonal Antibody, Unconjugated (ER1918-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-TRIM23 (ER1918-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Product Name | TRIM23 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP ribosylation factor domain protein 1 ARF domain protein 1 ARFD1 GTP binding protein ARD 1 TRI23_HUMAN N-acetyltransferase ARD1 human homolog of N-acetyltransferase homolog of S. cerevisiae ARD1 TRIM23 Tripartite motif protein TRIM23. |
| Product description | Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRIM23 201-300/574 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | ADP ribosylation factor domain protein 1 ARF domain protein 1 ARFD1 GTP binding protein ARD 1 TRI23_HUMAN N-acetyltransferase ARD1 human homolog of N-acetyltransferase homolog of S. cerevisiae ARD1 TRIM23 Tripartite motif protein TRIM23. |
|---|---|
| Molecular Weight(MW) | 64 KDa |
| Cellular Localization | Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Note: Membrane associated with the Golgi complex and lysosomal structures. |
| SwissProt ID | P36406 |
|---|
Application
Fig1: Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-TRIM23(ER1918-09)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig2: Sample: Hela (Human) Cell Lysate at 40 ug Primary: Anti-TRIM23(ER1918-09)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM23) Polyclonal Antibody, Unconjugated (ER1918-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-TRIM23 (ER1918-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
