Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated (ER1918-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti-TRIM68 (ER1918-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Product Name | TRIM68 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase TRIM68 FLJ10369 GC109 MGC126176 RING finger protein 137 RNF 137 RNF137 Ro/SSA1 related protein SS 56 SS-56 SS56 SSA protein SS 56 SSA protein SS-56 TRI68_HUMAN TRIM 68 Trim68 Tripartite motif containing 68 Tripartite motif containing protein 68 Tripartite motif-containing protein 68. |
| Product description | Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRIM68 201-300/485 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | E3 ubiquitin-protein ligase TRIM68 FLJ10369 GC109 MGC126176 RING finger protein 137 RNF 137 RNF137 Ro/SSA1 related protein SS 56 SS-56 SS56 SSA protein SS 56 SSA protein SS-56 TRI68_HUMAN TRIM 68 Trim68 Tripartite motif containing 68 Tripartite motif containing protein 68 Tripartite motif-containing protein 68. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus. |
| SwissProt ID | Q6AZZ1 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated (ER1918-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti-TRIM68 (ER1918-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Positive Control | Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
