Application
Fig1: Sample: Siha Cell (Human) Lysate at 40 ug Primary: Anti-TRIM7 (ER1918-12) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM7) Polyclonal Antibody, Unconjugated (ER1918-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | TRIM7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glycogenin-interacting protein GNIP RING finger protein 90 RNF90 TRIM7 TRIM7_HUMAN Tripartite Motif Containing 7 Tripartite motif protein TRIM 7 Tripartite motif-containing protein 7. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRIM7/RNF90 255-350/511 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Glycogenin-interacting protein GNIP RING finger protein 90 RNF90 TRIM7 TRIM7_HUMAN Tripartite Motif Containing 7 Tripartite motif protein TRIM 7 Tripartite motif-containing protein 7. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| SwissProt ID | Q9C029 |
|---|
Application
Fig1: Sample: Siha Cell (Human) Lysate at 40 ug Primary: Anti-TRIM7 (ER1918-12) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM7) Polyclonal Antibody, Unconjugated (ER1918-12) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Skeletal muscle and placenta, at lower levels in heart, brain and pancreas. Isoform 1 is widely expressed with high level in testis, kidney and heart. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
