Application
Fig1: Sample: Liver(Human) Cell Lysate at 40 ug Primary: Anti-TRPM5 (ER1918-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD| Product Name | TRPM5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLSN1 and TRP related gene 1 MLSN1 and TRP related gene 1 protein MLSN1 and TRP-related MLSN1- and TRP-related gene 1 protein MTR1 Novel protein similar to vertebrate transient receptor potential cation channel, subfamily M, member 5 Transient receptor potential cation channel subfamily M member 5 Transient receptor potential cation channel, subfamily M, member 5 Trpm5 TRPM5 transient receptor potential cation channel, subfamily M, member 5 TRPM5_HUMAN 9430099A16Rik Long transient receptor potential channel 5 LTrpC-5 LTrpC5. |
| Product description | Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. |
| Immunogen | KLH conjugated synthetic peptide derived from human TRPM5 701-800/1165 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | MLSN1 and TRP related gene 1
MLSN1 and TRP related gene 1 protein
MLSN1 and TRP-related
MLSN1- and TRP-related gene 1 protein
MTR1
Novel protein similar to vertebrate transient receptor potential cation channel subfamily M member 5 Transient receptor potential cation channel subfamily M member 5 Transient receptor potential cation channel subfamily M member 5 Trpm5 TRPM5 transient receptor potential cation channel subfamily M member 5 TRPM5_HUMAN 9430099A16Rik Long transient receptor potential channel 5 LTrpC-5 LTrpC5. |
|---|---|
| Molecular Weight(MW) | 131 KDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q9NZQ8 |
|---|
Application
Fig1: Sample: Liver(Human) Cell Lysate at 40 ug Primary: Anti-TRPM5 (ER1918-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD| Positive Control | Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
