Application
Fig1: Sample: Thyroid gland (Mouse) Lysate at 40 ug Primary: Anti-TSHR (ER1918-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kDApplication
Fig2: Sample: Thyroid gland (Rat) Lysate at 40 ug Primary: Anti-TSHR (ER1918-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD| Product Name | TSHR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hTSHR I hTSHRI LGR 3 LGR3 MGC75129 Thyroid adenoma hyperfunctioning Thyroid carcinoma with thyrotoxicosis Thyroid Stimulating Hormone Receptor Thyrotropin Receptor Thyrotropin receptor I TSH R TSHR Thyroid stimulating hormone receptor TSHR_HUMAN. |
| Product description | Various hormones are secreted from the anterior pituitary during development and growth, including thyroid-stimulating hormone (TSH, also known as thyrotropin), follicle-stimulating hormone (FSH) and leutinizing hormone (LH). TSH, FSH, and LH are heterodimers formed from a common alpha chain and a unique beta chain. TSH is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits. |
| Immunogen | KLH conjugated synthetic peptide derived from human TSHR 501-600/764 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | hTSHR I
hTSHRI
LGR 3 LGR3 MGC75129 Thyroid adenoma hyperfunctioning Thyroid carcinoma with thyrotoxicosis Thyroid Stimulating Hormone Receptor Thyrotropin Receptor Thyrotropin receptor I TSH R TSHR Thyroid stimulating hormone receptor TSHR_HUMAN. |
|---|---|
| Molecular Weight(MW) | 86 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P16473 |
|---|
Application
Fig1: Sample: Thyroid gland (Mouse) Lysate at 40 ug Primary: Anti-TSHR (ER1918-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD
Application
Fig2: Sample: Thyroid gland (Rat) Lysate at 40 ug Primary: Anti-TSHR (ER1918-20) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD| Positive Control | Expressed in the thyroid. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
