Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-TSPAN5 (ER1918-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-TSPAN5 (ER1918-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TSPAN5) Polyclonal Antibody, Unconjugated (ER1918-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TSPAN5) Polyclonal Antibody, Unconjugated (ER1918-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | TSPAN5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NET 4 NET-4 TSPAN 5 NET4 Tetraspan 5 Tetraspan NET 4 Tetraspan NET-4 Tetraspan TM4SF Tetraspanin 5 Tetraspanin-5 Tetraspanin5 TM4SF9 Transmembrane 4 superfamily member 8 Transmembrane 4 superfamily member 9 TSN5_HUMAN Tspan-5 TSPAN5. |
| Product description | DNA-binding protein that specifically binds heat shockpromoter elements (HSE) and activates transcription. In highereukaryotes, HSF is unable to bind to the HSE unless the cells areheat shocked. |
| Immunogen | KLH conjugated synthetic peptide derived from human TSPAN5 21-130/268 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | NET 4 NET-4 TSPAN 5 NET4 Tetraspan 5 Tetraspan NET 4 Tetraspan NET-4 Tetraspan TM4SF Tetraspanin 5 Tetraspanin-5 Tetraspanin5 TM4SF9 Transmembrane 4 superfamily member 8 Transmembrane 4 superfamily member 9 TSN5_HUMAN Tspan-5 TSPAN5. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein (Probable). |
| SwissProt ID | P62079 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-TSPAN5 (ER1918-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-TSPAN5 (ER1918-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TSPAN5) Polyclonal Antibody, Unconjugated (ER1918-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TSPAN5) Polyclonal Antibody, Unconjugated (ER1918-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
