Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (ER1918-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (ER1918-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-TUG (ER1918-28) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 58 kD| Product Name | TUG |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alveolar soft part sarcoma chromosomal region candidate gene 1 protein Alveolar soft part sarcoma chromosome region candidate 1 (human) Alveolar soft part sarcoma locus ASPC ASPC1_HUMAN ASPCR 1 ASPCR1 ASPL ASPS ASPSCR 1 Aspscr1 FLJ45380 RCC 17 RCC17 renal cell carcinoma gene on chromosome 17 renal cell carcinoma papillary 17 Renal papillary cell carcinoma protein 17 Tether containing a UBX domain for GLUT4 Tether containing UBX domain for GLUT4 TUG UBX domain containing protein 9 UBX domain protein 9 UBX domain-containing protein 9 UBXD 9 UBXD9 UBXN 9 UBXN9. |
| Product description | Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. |
| Immunogen | KLH conjugated synthetic peptide derived from human TUG/ASPC 351-420/553 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alveolar soft part sarcoma chromosomal region candidate gene 1 protein Alveolar soft part sarcoma chromosome region candidate 1 (human) Alveolar soft part sarcoma locus ASPC ASPC1_HUMAN ASPCR 1 ASPCR1 ASPL ASPS ASPSCR 1 Aspscr1 FLJ45380 RCC 17 RCC17 renal cell carcinoma gene on chromosome 17 renal cell carcinoma papillary 17 Renal papillary cell carcinoma protein 17 Tether containing a UBX domain for GLUT4 Tether containing UBX domain for GLUT4 TUG UBX domain containing protein 9 UBX domain protein 9 UBX domain-containing protein 9 UBXD 9 UBXD9 UBXN 9 UBXN9. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). |
| SwissProt ID | Q9BZE9 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (ER1918-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (ER1918-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-TUG (ER1918-28) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 58 kD| Positive Control | Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
