Application
Fig1: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-UBE2D2 (ER1918-30) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kDApplication
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-UBE2D2 (ER1918-30) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD| Product Name | UBE2D2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PUBC 1 PUBC1 UB2D2_HUMAN UBC 4 UBC 4/5 UBC4 UBC4/5 UBC4/5 homolog yeast UBCH 5B UBCH5B UBE2D2 Ubiquitin carrier protein Ubiquitin carrier protein D2 Ubiquitin conjugating enzyme E2 17 kDa 2 Ubiquitin conjugating enzyme E2 D2 Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 2 Ubiquitin protein ligase D2 Ubiquitin-conjugating enzyme E2 D2 Ubiquitin-conjugating enzyme E2(17)KB 2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-protein ligase D2 E2(17)KB2. |
| Product description | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. |
| Immunogen | KLH conjugated synthetic peptide derived from human UBE2D2 31-130/147 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | PUBC 1 PUBC1 UB2D2_HUMAN UBC 4 UBC 4/5 UBC4 UBC4/5 UBC4/5 homolog yeast UBCH 5B UBCH5B UBE2D2 Ubiquitin carrier protein Ubiquitin carrier protein D2 Ubiquitin conjugating enzyme E2 17 kDa 2 Ubiquitin conjugating enzyme E2 D2 Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 2 Ubiquitin protein ligase D2 Ubiquitin-conjugating enzyme E2 D2 Ubiquitin-conjugating enzyme E2(17)KB 2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-protein ligase D2 E2(17)KB2. |
|---|---|
| Molecular Weight(MW) | 17 KDa |
| SwissProt ID | P62837 |
|---|
Application
Fig1: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-UBE2D2 (ER1918-30) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD
Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-UBE2D2 (ER1918-30) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
