Application
Fig1: Sample: Embryo (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-UBOX5 (ER1918-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD| Product Name | UBOX5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UIP5 UIP-5 UIP 5 RING finger protein 37 RNF37 U-box domain containing 5 U-box domain-containing protein 5 UBCE7IP5 ubiquitin conjugating enzyme 7 interacting protein 5 UBOX5 RNF37_HUMAN. |
| Product description | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. |
| Immunogen | KLH conjugated synthetic peptide derived from human UBOX5/RNF37 21-120/541 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | UIP5 UIP-5 UIP 5 RING finger protein 37 RNF37 U-box domain containing 5 U-box domain-containing protein 5 UBCE7IP5 ubiquitin conjugating enzyme 7 interacting protein 5 UBOX5 RNF37_HUMAN. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Nucleus. Note=Associated with chromatin during meiosis. |
| SwissProt ID | O94941 |
|---|
Application
Fig1: Sample: Embryo (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-UBOX5 (ER1918-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD| Positive Control | Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
