Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (ER1918-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UBXD2 (ER1918-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (ER1918-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | UBXD2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Erasin FLJ23318 KIAA0242 KIAA2042 UBX domain containing 1 UBX domain containing 2 UBX domain containing protein 2 UBXD 2 UBXDC 1 UBXDC1 UBXN4_HUMAN. |
| Product description | UBXD2 is an integral membrane protein of the endoplasmic reticulum (ER) that binds valosin-containing protein (VCP) and promotes ER-associated protein degradation (ERAD). Immunohistochemical staining of brain tissue from patients with Alzheimer's disease and control subjects revealed that UBXD2 accumulates preferentially in neurons undergoing neurofibrillary degeneration in Alzheimer's disease. |
| Immunogen | KLH conjugated synthetic peptide derived from human UBXD2 201-300/508 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Erasin FLJ23318 KIAA0242 KIAA2042 UBX domain containing 1 UBX domain containing 2 UBX domain containing protein 2 UBXD 2 UBXDC 1 UBXDC1 UBXN4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Endoplasmic reticulum membrane. Both the N- and the C-terminus face the cytosol; also found in the nucleus envelope contiguous to the ER. |
| SwissProt ID | Q92575 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (ER1918-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UBXD2 (ER1918-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (ER1918-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Accumulates in Alzheimer disease-afflicted brains (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
