Application
Fig1: Blank control: Mouse spleen (blue). Primary Antibody:Rabbit Anti-UCP-1 antibody(ER1918-37), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1918-37, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- UCP-1 (ER1918-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Product Name | UCP-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Uncoupling Protein-1 UCP1 UCP 1 Mitochondrial brown fat uncoupling protein 1 mitochondrial brown fat uncoupling protein SLC25A7 Thermogenin UCP 1 UCP Uncoupling protein 1 UCP1_HUMAN. |
| Product description | Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria. |
| Immunogen | KLH conjugated synthetic peptide derived from human UCP-1 101-200/307 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Uncoupling Protein-1 UCP1 UCP 1 Mitochondrial brown fat uncoupling protein 1 mitochondrial brown fat uncoupling protein SLC25A7 Thermogenin UCP 1 UCP Uncoupling protein 1 UCP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. |
| SwissProt ID | P12242 |
|---|
Application
Fig1: Blank control: Mouse spleen (blue). Primary Antibody:Rabbit Anti-UCP-1 antibody(ER1918-37), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1918-37, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti- UCP-1 (ER1918-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Positive Control | Brown adipose tissue, Mouse spleen, Thymus (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
