Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug HT1080(Human) Cell Lysate at 30 ug Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- UNC5B (ER1918-41) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kD Observed band size: 111 kD| Product Name | UNC5B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UNC5H2 Netrin receptor UNC5B Netrin receptor UNC5B Precursor p53 regulated receptor for death and life p53 regulated receptor for death and life protein 1 p53-regulated receptor for death and life protein 1 p53RDL1 Protein unc-5 homolog 2 Protein unc-5 homolog B Protein XUNC 5 Transmembrane receptor Unc5H2 Unc 5 homolog 2 Unc 5 homolog B UNC 5B Unc5 (C.elegans homolog) b Unc5b UNC5B_HUMAN UNC5H2. |
| Product description | FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human UNC5B 188-260/945 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | UNC5H2 Netrin receptor UNC5B Netrin receptor UNC5B Precursor p53 regulated receptor for death and life p53 regulated receptor for death and life protein 1 p53-regulated receptor for death and life protein 1 p53RDL1 Protein unc-5 homolog 2 Protein unc-5 homolog B Protein XUNC 5 Transmembrane receptor Unc5H2 Unc 5 homolog 2 Unc 5 homolog B UNC 5B Unc5 (C.elegans homolog) b Unc5b UNC5B_HUMAN UNC5H2. |
|---|---|
| Molecular Weight(MW) | 101 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Note=Associated with lipid raft. |
| SwissProt ID | Q8IZJ1 |
|---|
Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug HT1080(Human) Cell Lysate at 30 ug Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- UNC5B (ER1918-41) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kD Observed band size: 111 kD| Positive Control | Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
