Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-UNCX (ER1918-42) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UNCX) Polyclonal Antibody, Unconjugated (ER1918-42) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig6: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 55 kD| Product Name | UNCX |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein unc-4 homolog Homeobox protein Uncx4.1 tcag7.1272 UNC homeobox Unc4.1 homeobox UNC4_HUMAN Uncx UNCX4.1. |
| Product description | Transcription factor involved in somitogenesis and neurogenesis. Required for the maintenance and differentiation of particular elements of the axial skeleton. May act upstream of PAX9. Plays a role in controlling the development of connections of hypothalamic neurons to pituitary elements, allowing central neurons to reach the peripheral blood circulation and to deliver hormones for control of peripheral functions. |
| Immunogen | KLH conjugated synthetic peptide derived from human UNCX 145-150/531 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPig |
| Concentration | 1mg/ml |
| Alternative Names | Homeobox protein unc-4 homolog Homeobox protein Uncx4.1 tcag7.1272 UNC homeobox Unc4.1 homeobox UNC4_HUMAN Uncx UNCX4.1. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | A6NJT0 |
|---|
Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-UNCX (ER1918-42) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (UNCX) Polyclonal Antibody, Unconjugated (ER1918-42) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig6: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-UNCX (ER1918-42) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 55 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
