Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti- Vasohibin 1 (ER1918-47) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kDApplication
Fig2: Sample: Liver（Rat） lysate at 30ug; Primary: Anti-alpha-Actinin-4 (ER1918-47) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution; Predicted band size : 40kD Observed band size : 40kD| Product Name | Vasohibin 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vasohibin KIAA1036 VASH VASH VASH1 VASH1_HUMAN Vasohibin1 Vasohibin-1. |
| Product description | Angiogenesis inhibitor. Inhibits migration,proliferation and network formation by endothelial cells as well asangiogenesis. This inhibitory effect is selective to endothelialcells as it does not affect the migration of smooth muscle cells orfibroblasts. Does not affect the proliferation of cancer cells invitro, but inhibits tumor growth and tumor angiogenesis. Acts in anautocrine manner. Inhibits artery neointimal formation andmacrophage infiltration. Exhibits heparin-binding activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human VASH1 281-365/365 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Vasohibin KIAA1036 VASH VASH VASH1 VASH1_HUMAN Vasohibin1 Vasohibin-1. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q7L8A9 |
|---|
Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti- Vasohibin 1 (ER1918-47) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD
Application
Fig2: Sample: Liver（Rat） lysate at 30ug; Primary: Anti-alpha-Actinin-4 (ER1918-47) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution; Predicted band size : 40kD Observed band size : 40kD| Positive Control | Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
