Application
Fig1: Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kDApplication
Fig2: Sample: BRL-3A Cell (Rat) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kDApplication
Fig3: Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kDApplication
Fig4: Sample: MCF-7 Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 36 kDApplication
Fig5: Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-VAX1 antibody (ER1918-48),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Product Name | VAX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VAX1 VAX1_HUMAN ventral anterior homeobox 1. |
| Product description | Required for axon guidance and major tract formation in the developing forebrain. May contribute to the differentiation of the neuroretina, pigmented epithelium and optic stalk. |
| Immunogen | KLH conjugated synthetic peptide derived from human VAX1 133-200/334 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:0.2μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | VAX1 VAX1_HUMAN ventral anterior homeobox 1. |
|---|---|
| Molecular Weight(MW) | 35 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q5SQQ9 |
|---|
Application
Fig1: Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD
Application
Fig2: Sample: BRL-3A Cell (Rat) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD
Application
Fig3: Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug Primary: Anti- VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD
Application
Fig4: Sample: MCF-7 Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-VAX1 (ER1918-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 36 kD
Application
Fig5: Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃). Primary Antibody (green line): Rabbit Anti-VAX1 antibody (ER1918-48),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Application Notes | WB:1:500-2000: FC:0.2μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
