Application
Fig1: Sample: Lane1:Brain(Rat) Lysate at 30 ug Lane2:Liver (Rat) Lysate at 30 ug Primary: Anti-VISFATIN-1 (ER1918-53) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 55kD Observed band size : 54kDApplication
Fig2: Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(ER1918-53) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(ER1918-53) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Visfatin (ER1918-53) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | Visfatin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | pre-B-cell colony-enhancing factor 1 PBEF1 protein AI480535 1110035O14Rik AI314458 DKFZP666B131 EC 2.4.2.12 MGC117256 NAmPRTase NAMPT Nicotinamide phosphoribosyltransferase PBEF PBEF1 Pre B cell colony enhancing factor 1 Pre B cell colony enhancing factor Pre B cell enhancing factor Visfatin NAMPT_HUMAN. |
| Product description | Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. |
| Immunogen | KLH conjugated synthetic peptide derived from human PBEF 301-400/491 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseMonkey |
| Concentration | 1mg/ml |
| Alternative Names | pre-B-cell colony-enhancing factor 1 PBEF1 protein AI480535 1110035O14Rik AI314458 DKFZP666B131 EC 2.4.2.12 MGC117256 NAmPRTase NAMPT Nicotinamide phosphoribosyltransferase PBEF PBEF1 Pre B cell colony enhancing factor 1 Pre B cell colony enhancing factor Pre B cell enhancing factor Visfatin NAMPT_HUMAN. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P43490 |
|---|
Application
Fig1: Sample: Lane1:Brain(Rat) Lysate at 30 ug Lane2:Liver (Rat) Lysate at 30 ug Primary: Anti-VISFATIN-1 (ER1918-53) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 55kD Observed band size : 54kD
Application
Fig2: Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(ER1918-53) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(ER1918-53) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Visfatin (ER1918-53) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
