Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-VNN1 antibody (ER1918-55) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: placenta(Mouse) Lysate at 40 ug Primary: Anti-VNN1(ER1918-55) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig3: Blank control: 293T(blue). Primary Antibody:Rabbit Anti-VNN1 antibody(ER1918-55), Dilution: 5μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were washed twice with phosphate-buffered saline (PBS).The cells were then incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (ER1918-55, 5μg /1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig4: Blank control: 293T. Primary Antibody (green line): Rabbit Anti-VNN1 antibody (ER1918-55) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | VNN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDLCQ8 High density lipoprotein cholesterol level quantitative trait locus 8, included Pantetheinase Pantetheine hydrolase Tiff66 V-1 antibody Vanin 1 Vanin-1 Vannin 1 Vascular non inflammatory molecule 1 Vascular non-inflammatory molecule 1 VNN 1 VNN1 VNN1_HUMAN. |
| Product description | Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. |
| Immunogen | KLH conjugated synthetic peptide derived from human VNN1 151-250/513 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:2μg/Test: |
|
| Species Reactivity | HumanMouseRatCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | HDLCQ8
High density lipoprotein cholesterol level quantitative trait locus 8 included Pantetheinase Pantetheine hydrolase Tiff66 V-1 antibody Vanin 1 Vanin-1 Vannin 1 Vascular non inflammatory molecule 1 Vascular non-inflammatory molecule 1 VNN 1 VNN1 VNN1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor |
| SwissProt ID | O95497 |
|---|
Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-VNN1 antibody (ER1918-55) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: placenta(Mouse) Lysate at 40 ug Primary: Anti-VNN1(ER1918-55) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig3: Blank control: 293T(blue). Primary Antibody:Rabbit Anti-VNN1 antibody(ER1918-55), Dilution: 5μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were washed twice with phosphate-buffered saline (PBS).The cells were then incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (ER1918-55, 5μg /1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig4: Blank control: 293T. Primary Antibody (green line): Rabbit Anti-VNN1 antibody (ER1918-55) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin. |
|---|---|
| Application Notes | WB:1:500-2000: FC:2μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
