Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (ER1918-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (ER1918-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig3: Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- VSIG4 Polyclonal Antibody, Unconjugated(ER1918-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: Lung(Mouse)Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Primary: Anti-VSIG4 (ER1918-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Product Name | VSIG4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | V-set and immunoglobulin domain-containing protein 4 CRIg V set and immunoglobulin domain containing 4 Z39IG VSIG4_HUMAN. |
| Product description | Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. |
| Immunogen | KLH conjugated synthetic peptide derived from human VSIG4 81-160/399 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | V-set and immunoglobulin domain-containing protein 4 CRIg V set and immunoglobulin domain containing 4 Z39IG VSIG4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Probable). |
| SwissProt ID | Q9Y279 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (ER1918-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (ER1918-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig3: Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- VSIG4 Polyclonal Antibody, Unconjugated(ER1918-60) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: Lung(Mouse)Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Primary: Anti-VSIG4 (ER1918-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Positive Control | Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
