Application
Fig1: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(ER1918-63) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(ER1918-63) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).Application
Fig4: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-WDR26 (ER1918-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD| Product Name | WDR26 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | myocardial ischemic preconditioning upregulated protein 2 WDR26 PRO0852 CDW2 MIP2 CUL4 and DDB1 associated WDR protein 2 Myocardial ischemic preconditioning up regulated protein 2 myocardial ischemic preconditioning upregulated protein 2 WD repeat domain 26 WD repeat-containing protein 26 FLJ21016 WDR26_HUMAN. |
| Product description | May be involved in MAPK pathways. |
| Immunogen | KLH conjugated synthetic peptide derived from human WDR26 101-200/514 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | myocardial ischemic preconditioning upregulated protein 2 WDR26 PRO0852 CDW2 MIP2 CUL4 and DDB1 associated WDR protein 2 Myocardial ischemic preconditioning up regulated protein 2 myocardial ischemic preconditioning upregulated protein 2 WD repeat domain 26 WD repeat-containing protein 26 FLJ21016 WDR26_HUMAN. |
|---|---|
| Molecular Weight(MW) | 72 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9H7D7 |
|---|
Application
Fig1: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(ER1918-63) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(ER1918-63) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig4: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-WDR26 (ER1918-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD| Positive Control | Broadly expressed, with highest levels in heart and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
