Application
Fig1: Sample: Heart (Rat) Lysate at 40 ug Primary: Anti-WEE1 (ER1918-64) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 72 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (ER1918-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | WEE1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686I18166 EC 2.7.10.2 FLJ16446 Wee 1 WEE-1 WEE1 protein Wee1 homolog WEE1 homolog S. pombe Wee1 like protein kinase Wee1 tyrosine kinase Wee1+ homolog Wee1+ S. pombe homolog WEE1A Wee1A kinase WEE1hu WEE1 tyrosine kinase isoform 1 MGC105683 WEE1_HUMAN. |
| Product description | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation |
| Immunogen | KLH conjugated synthetic peptide derived from human WEE1 151-250/646 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686I18166 EC 2.7.10.2 FLJ16446 Wee 1 WEE-1 WEE1 protein Wee1 homolog WEE1 homolog S. pombe Wee1 like protein kinase Wee1 tyrosine kinase Wee1+ homolog Wee1+ S. pombe homolog WEE1A Wee1A kinase WEE1hu WEE1 tyrosine kinase isoform 1 MGC105683 WEE1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 72 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P30291 |
|---|
Application
Fig1: Sample: Heart (Rat) Lysate at 40 ug Primary: Anti-WEE1 (ER1918-64) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 72 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (ER1918-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
