Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (ER1918-66) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (ER1918-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kDApplication
Fig3: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (ER1918-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD| Product Name | WISP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTGFL CCN family member 5 CCN5 Connective tissue growth factor like protein Connective tissue growth factor related protein 58 Connective tissue growth factor-like protein Connective tissue growth factor-related protein 58 CT58 CTGF L CTGFL CTGF-L WISP 2 WISP-2 Wisp2 WISP2_HUMAN WNT1 inducible signaling pathway protein 2 WNT1-inducible-signaling pathway protein 2. |
| Product description | May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. |
| Immunogen | KLH conjugated synthetic peptide derived from human CTGFL 171-250/2502 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | CTGFL CCN family member 5 CCN5 Connective tissue growth factor like protein Connective tissue growth factor related protein 58 Connective tissue growth factor-like protein Connective tissue growth factor-related protein 58 CT58 CTGF L CTGFL CTGF-L WISP 2 WISP-2 Wisp2 WISP2_HUMAN WNT1 inducible signaling pathway protein 2 WNT1-inducible-signaling pathway protein 2. |
|---|---|
| Molecular Weight(MW) | 24 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O76076 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (ER1918-66) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (ER1918-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD
Application
Fig3: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-WISP2 (ER1918-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD| Positive Control | Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
