Application
Fig1: Sample: Eye(Mouse) Lysate at 30 ug Primary: Anti- WNK4 (ER1918-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 135 kD Observed band size: 135 kD| Product Name | WNK4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHA 2B PHA2B PRKWNK 4 PRKWNK4 Protein kinase lysine deficient 4 Protein kinase with no lysine 4 Serine/threonine protein kinase WNK4 WNK lysine deficient protein kinase 4 WNK4_HUMAN. |
| Product description | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. |
| Immunogen | KLH conjugated synthetic peptide derived from human WNK4 351-450/1243 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | PHA 2B PHA2B PRKWNK 4 PRKWNK4 Protein kinase lysine deficient 4 Protein kinase with no lysine 4 Serine/threonine protein kinase WNK4 WNK lysine deficient protein kinase 4 WNK4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 135 KDa |
| Cellular Localization | Cell junction, tight junction (By similarity). Note=Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity). |
| SwissProt ID | Q96J92 |
|---|
Application
Fig1: Sample: Eye(Mouse) Lysate at 30 ug Primary: Anti- WNK4 (ER1918-67) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 135 kD Observed band size: 135 kD| Positive Control | Expressed in kidney, colon and skin. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
