Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 34 kDApplication
Fig2: Sample: Embryo (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (ER1918-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 63 kD| Product Name | Wnt11 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HWNT11 Protein Wnt-11 Wingless-related MMTV integration site 11 Wingless-type MMTV integration site family, member 1 Wingless-type MMTV integration site family, member 11 Wingless-type MMTV integration site family, member 11B wnt-11 wnt11b.WNT11_HUMAN |
| Product description | Wnt11 is a non-canonical Wnt which is expressed in androgen-independent prostate cancer cell lines and prostate tumours. It is involved in development, including cardiac and gut development. |
| Immunogen | KLH conjugated synthetic peptide derived from human Wnt11 151-250/354 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | HWNT11
Protein Wnt-11
Wingless-related MMTV integration site 11
Wingless-type MMTV integration site family member 1 Wingless-type MMTV integration site family member 11 Wingless-type MMTV integration site family member 11B wnt-11 wnt11b.WNT11_HUMAN |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Secreted; extracellular space; extracellular matrix. |
| SwissProt ID | O96014 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 34 kD
Application
Fig2: Sample: Embryo (Mouse) Lysate at 40 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (ER1918-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Wnt11 (ER1918-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 63 kD| Positive Control | Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
