Application
Fig1: Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated (ER1918-70) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.Application
Fig2: Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-Wnt6 (ER1918-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kDApplication
Fig3: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Wnt6 (ER1918-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD| Product Name | Wnt6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Wnt-6 precursor WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY, MEMBER 6 wnt 6 WNT6_HUMAN. |
| Product description | Wingless-type MMTV integration site family, member 6, also known as WNT6, is a human gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, which are involved in the Wnt signaling pathway. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. Wnt6 plays a role in the formation and maturation of different embryonic structures, namely the fetal heart, ventral body wall, and somite derived structures. Wnt6, through the canonical Wnt signaling pathway, inhibits the induction of cardiogenic mesoderm. On the other hand, if Wnt6 is upregulated, muscle in the limbs and surrounding areas are decreased as the mesenchymal progenitor cells that migrate and become the muscle are locked in the somite as epithelial cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human Wnt6 281-365/365 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Protein Wnt-6 precursor
WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER 6 wnt 6 WNT6_HUMAN. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q9Y6F9 |
|---|
Application
Fig1: Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated (ER1918-70) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.
Application
Fig2: Sample: MKN45(Human) Cell Lysate at 30 ug Primary: Anti-Wnt6 (ER1918-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kD
Application
Fig3: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-Wnt6 (ER1918-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD| Positive Control | Human breast carcinom, MKN45(Human) Cell Lysate, NIH/3T3(Mouse) Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
