Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated (ER1918-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-WNT7A (ER1918-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD| Product Name | WNT7A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Wnt-7a wnt 7a Protein Wnt-7a precursor proto-oncogene wnt7a protein wingless-type MMTV integration site family, member 7A WNT7A WNT7A_HUMAN. |
| Product description | Protein Wnt-7a is a protein that in humans is encoded by the WNT7A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein showing 99% amino acid identity to the mouse Wnt7A protein. This gene not only guides the development of the anterior-posterior axis in the female reproductive tract but also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. It is also responsive to changes in the levels of sex steroid hormone in the female reproductive tract. Decreased expression of this gene in human uterine leiomyoma is found to be inversely associated with the expression of estrogen receptor alpha. |
| Immunogen | KLH conjugated synthetic peptide derived from human WNT7A 241-349/349 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Protein Wnt-7a
wnt 7a
Protein Wnt-7a precursor proto-oncogene wnt7a protein wingless-type MMTV integration site family member 7A WNT7A WNT7A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | O00755 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated (ER1918-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-WNT7A (ER1918-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD| Positive Control | Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
