Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- WRCH1 (ER1918-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Product Name | WRCH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WRCH-1 WRCH 1 Wnt-1 responsive Cdc42 homolog 1 Rho-related GTP-binding protein RhoU Ryu GTPase CDC42-like GTPase 1 Rho GTPase-like protein ARHU GTP-binding protein-like 1 RHOU SB128 ARHU CDC42L1 G28K WRCH1 RHOU_HUMAN. |
| Product description | Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. |
| Immunogen | KLH conjugated synthetic peptide derived from human WRCH-1 151-258/258 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | WRCH-1 WRCH 1 Wnt-1 responsive Cdc42 homolog 1 Rho-related GTP-binding protein RhoU Ryu GTPase CDC42-like GTPase 1 Rho GTPase-like protein ARHU GTP-binding protein-like 1 RHOU SB128 ARHU CDC42L1 G28K WRCH1 RHOU_HUMAN. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction, focal adhesion. Cell projection, podosome. Note=Localizes to podosomes in SRC-transformed cells. |
| SwissProt ID | Q7L0Q8 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- WRCH1 (ER1918-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Positive Control | Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
