Application
Fig1: Sample:A549 (Human)cell Lysate at 40 ug Primary: Anti-ZAR1L(ER1918-79)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated (ER1918-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | ZAR1L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RP11-37E23.4 ZAR1-like protein ZAR1L ZAR1L_HUMAN ZAR2 Zygote arrest 1 like. |
| Immunogen | KLH conjugated synthetic peptide derived from human ZAR1L 51-150/321 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | RP11-37E23.4 ZAR1-like protein ZAR1L ZAR1L_HUMAN ZAR2 Zygote arrest 1 like. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| SwissProt ID | A6NP61 |
|---|
Application
Fig1: Sample:A549 (Human)cell Lysate at 40 ug Primary: Anti-ZAR1L(ER1918-79)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated (ER1918-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
